human ccl21 6ckine antibody  (R&D Systems)

Journal: Scientific Reports

Article Title: Immune regulation and lymphangiogenesis by lymphatic endothelial cells in the decidua in severe preeclampsia

doi: 10.1038/s41598-026-35667-3

Figure Lengend Snippet: Reduced CCL21 expression and impaired dendritic cell (DC) migration in PE-dLECs. ( A ) PE-dLECs showed significantly reduced expression of CCL21 at both the mRNA and protein levels, as assessed using RT-qPCR and western blot, respectively. ( B ) DC trafficking through LECs was evaluated in three steps: migration, adhesion, and transmigration. PE-dLECs exhibited reduced activity in all three steps. ( C ) Decreased DC migration observed with conditioned media from PE-dLECs was restored by the addition of recombinant CCL21. Data are presented as mean ± SEM. Statistical significance was assessed using one-way ANOVA followed by Tukey’s test. All experiments represent three independent experiments, each performed in duplicate. **, p < 0.01 vs. N-dLEC. N-dLECs, dLECs from normal pregnancies; PE-dLECs, dLECs from preeclamptic pregnancies; DC, dendritic cell; dLECs, decidua lymphatic endothelial cells; PE, preeclampsia; CCL 21, chemokine (C-C motif) ligand 21; RT-qPCR, quantitative reverse-transcription PCR.

Article Snippet: Conditioned media from PE or normal pregnancies collected after 24 h of culture in EBM with 1% FBS were added to the lower chamber, with or without recombinant human CCL21 (250 ng/mL; R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Migration, Quantitative RT-PCR, Western Blot, Transmigration Assay, Activity Assay, Recombinant, Reverse Transcription

Journal: Scientific Reports

Article Title: Immune regulation and lymphangiogenesis by lymphatic endothelial cells in the decidua in severe preeclampsia

doi: 10.1038/s41598-026-35667-3

Figure Lengend Snippet: Proposed model of immune and lymphatic dysfunction at the maternal-fetal interface in preeclampsia. This schematic illustrates the functional and genetic differences observed in PE-dLECs. In PE-dLECs, reduced expression of lymphangiogenesis-related genes leads to impaired lymphatic vessel formation and functional deficits. Decreased CCL21 expression disrupts DC trafficking, while reduced NO levels impair the suppression of cytotoxic T cells. Finally, these changes may contribute to immune imbalance and the pathogenesis of preeclampsia. LV, lymphatic vessel; BV, blood vessel; M, amnion chorion; D, decidua; dLECs, decidual lymphatic endothelial cells; PE, pre-eclampsia; NO, nitric oxide; CCL 21, chemokine (C-C motif) ligand 21; DC, dendritic cell.

Article Snippet: Conditioned media from PE or normal pregnancies collected after 24 h of culture in EBM with 1% FBS were added to the lower chamber, with or without recombinant human CCL21 (250 ng/mL; R&D Systems, Minneapolis, MN, USA).

Techniques: Functional Assay, Expressing

Journal: PLOS One

Article Title: Bulk and single-cell RNA-sequencing analyses revealed potential key genes and the role of CCL19/CCL21-CCR7 axis in hidradenitis suppurativa

doi: 10.1371/journal.pone.0322565

Figure Lengend Snippet: A. CCR7 gene expression in HS lesion. B. Serum levels of CCL19 and CCL21 (n = 8). C. Correlation analysis between CCL19 and CCL21 in the skin and Treg cells in the blood. D. HS and healthy control average gene expression of Treg cluster. E. Flow cytometric analysis of CCR7 + lymphocytes percentages within lymphocytes compartment and Treg percentages within the CD4 + T cell compartment (n = 3). F. The CLL19 and CLL21 genes expression analysis in the HS cell subset clusters. (*P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001).

Article Snippet: Plasma levels of CCL19 (BOSTER, EK0456) and CCL21 (BOSTER, EK0555) were quantified using a commercially available ELISA kits according to the manufacturer’s protocol.

Techniques: Gene Expression, Control, Expressing